As mRNA-based therapeutics gain momentum, precise characterization of their critical quality attributes (CQAs) becomes essential. Whether you are looking for high-throughput solutions or need to uncover deeper structural insights, Agilent has the right tools for your mRNA analysis.

Our webinar kicks off with capillary electrophoresis (CE) systems —your solution for fast, automated QC— to analyze size, quantity, and integrity of your samples. These automated platforms provide high-throughput and reliable quality control (QC) of your mRNA, ensuring that IVT reactions are optimized, screening polyA tail length and integrity, and assessing the IVT final product is stable.

We then dive deeper into mRNA structure with LC/MS mapping — your go-to workflow for detailed analysis. This methodology offers valuable insights into primary structural properties including mRNA sequence, 3' poly(A) tail length, 5' capping efficiency, PTMs and sequence variants. Plus, our deactivated stainless-steel columns tackle common LC issues like adsorption and metal leaching, delivering consistent, reliable results every time.

Join us to discover how Agilent’s integrated solutions streamline mRNA CQA analysis from fragment profiling to detailed structural mapping!

By attending this webinar you will:

• Understand CQAs for mRNA therapeutics and their role in ensuring safety, efficacy, and stability.
• Learn high-throughput QC methods using capillary electrophoresis for size, quantity, and integrity analysis.
• Explore LC/MS mapping for sequence confirmation, poly(A) tail length, capping efficiency, PTMs, and variants.
• Reduce LC issues with deactivated stainless-steel columns for consistent, reliable results.
• Discover integrated workflows that streamline mRNA analysis from fragment profiling to structural mapping.

Speakers:

Charlotte Lekieffre Ph.D
BioPharma Application Engineer
Agilent Technologies

James Cameron
Market Specialist - BioPharma
Agilent Technologies