Simple Signaling Reporter Assays – Easily Quantify Activation & Inhibition of Cellular Pathways
Date: Wednesday, June 17, 2020
Time: 9:00am PDT / 12:00pm EDT
Duration: 30 minutes with additional time for Q&A
Cell-based reporter gene assays are a well-established screening modality for developing drugs that target diverse signaling pathways. In this webinar, we will introduce new PathHunter® Signaling Pathway Reporter Assays which utilize the industry-validated Enzyme Fragment Complementation (EFC) technology to quantify reporter gene activity. These pathway signaling reporter assays are robust, sensitive, and easy-to-use. We will provide examples of PathHunter reporter genes assays that quantify activation and inhibition of several different signaling pathways (e.g. NF-κB, NFAT and STAT3) via endogenous or heterologously introduced target receptors, such as CD40, RANK and IL-6R. We will also contrast data from two different but complementary assays targeting the inhibitory checkpoint receptor PD-1. One assay, the PathHunter PD1 Signaling asssay, measures ligand-dependent SHP1 recruitment, an early event in ligand-dependent PD-1 signaling, while the PD-1 Pathway Reporter Assay provides a more distal readout via activation of an NFAT reporter.
The webinar will discuss:
- Robust, sensitive, & easy-to-use cell-based, reporter gene assays to study signaling pathways
- Assay applications to quantify activation & inhibition of several different pathways
- Comparison of inhibitory checkpoint receptor PD-1 functional data from 2 different cell-based assay types – distal reporter based assays vs proximal receptor signaling assays
Speaker:
Jennifer Lin-Jones, Ph.D.
Senior Group Leader, Assay Development
Eurofins DiscoverX